Variable Radiobiological Responses of Spheroids
- 1 January 1980
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 81 (1), 85-99
- https://doi.org/10.2307/3575364
Abstract
Several techniques are now available for the growth of mammalian cells in culture as 3-dimensional spheroids. Use of these spheroids as in vitro models of solid tumors is increasing, yet little characterization of most systems, other than histological appearance, has been forthcoming. Spheroids produced with different sublines of V79 Chinese hamster cells may exhibit vastly different radiobiological properties, despite having a similar histological appearance. Quantitative characterization of the radiation response of these spheroids indicates that the responses are highly dependent upon cell type, whether spherhoids are irradiated in stirred or unstirred cultures and the techniques used to recover cells from spheroids. The extent and/or degree of hypoxia within spheroids does not always correlate with spheroid radiosensitivity or development of central necrosis. The necessity of adequately characterizing such model systems were indicated and results obtained with 1 spheroid system apparently cannot be used as a baseline for another.This publication has 11 references indexed in Scilit:
- Oxygen Diffusion Distance and Development of Necrosis in Multicell SpheroidsRadiation Research, 1979
- Physical and nutritional factors in gel culture of mammalian cellsIn Vitro Cellular & Developmental Biology - Plant, 1978
- GROWTH FRACTION AS MAJOR DETERMINANT OF MULTICELLULAR TUMOR SPHEROID GROWTH-RATES1978
- PROLIFERATION AND VIABILITY IN CELLULAR SPHEROIDS OF HUMAN ORIGIN1978
- A proliferation gradient in three‐dimensional colonies of cultured human glioma cellsInternational Journal of Cancer, 1977
- THE FINE STRUCTURE OF THREE-DIMENSIONAL COLONIES OF HUMAN GLIOMA CELLS IN AGAROSE CULTUREActa Pathologica Microbiologica Scandinavica Section A Pathology, 1977
- A New Method of Determining the Fraction of Hypoxic Cells in a Transplantable Murine SarcomaRadiation Research, 1977
- SIMPLIFIED METHOD FOR PRODUCTION AND GROWTH OF MULTICELLULAR TUMOR SPHEROIDS1977
- CELL CYCLE KINETICS IN AN IN VITRO TUMOR MODELCell Proliferation, 1976
- Colonial Growth in Agar of Cells Derived From Adenovirus-Induced Hamster Tumors2JNCI Journal of the National Cancer Institute, 1967