Protective effect of anionic cholecystographic agents against phalloidin on isolated hepatocytes by competive inhibition of the phallotoxin uptake

Abstract

Several anionic substances used for cholecystography inhibit the development of protrusions in isolated hepatocytes in response to phalloidin. Drugs from the iopodate family were equieffective with those of the iodipamide type. The above protective effect results from a competitive inhibition of the phallotoxin uptake as shown for iopodate. Cholecystographic agents similarily inhibit the inward transport of cholic acid in a competitive manner. The inhibition of the phallotoxin response is inversely correlated with the uptake of ³H-demethylphalloin (r=0.94) and with the inward transport of cholate (r=0.84) at various inhibiting concentrations of iopodate.