Kovalente, C-terminale Hydrophilisierung von Bromcyanpeptiden mit Arginin im Sequenator

Abstract

Cyanogen bromide peptides are modified with arginine (free base) at the C-terminal homoserine lactone. This modification makes the peptides more hydrophilic. In that way the degradation is possible up to homoserine and the C-terminal arginine. The phenylthiohydantoin derivatives of homoserine and arginine can be detected in good yields by TLC or high performance liquid chromatography. The automatic Edman degradation is investigated under various conditions.