Recording Integrating Photoelectric and Radioactive Scanner for Paper Electrophoresis and Chromatography

Abstract

A versatile automatic scanner for paper strips which records directly optical density or radioactivity is built around a servo type recorder (Brown). Interference filters provide a narrow band light source. For scanning in the visible or ultraviolet where monochromatic light is desired the monochromator of a Beckman model DU spectrophotometer replaces the filters. The instrument incorporates positive synchronization of strip and recording paper, sequential scanning of strips to provide an optical density curve followed by superposition of the integral of this curve. Areas are easily calculated without the need for planimetry. The slits of varying size are interchangeable and are designed for either optical or radioactive scanning. For medium levels of activity a Geiger‐Müller tube is interchangeable with the photocell, while for low activities, optical scanning of autoradiographs may be performed.