Structure and topology of 16S ribosomal RNA. An analysis of the pattern of psoralen crosslinking
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (8), 1855-1872
- https://doi.org/10.1093/nar/8.8.1855
Abstract
All of the eleven psoralen crosslinking features previously mapped by electron microscopy of the 16S rRNA can be matched by energetically stable duplex regions found by a computer search of the sequence. One additional particularly stable duplex found in the sequence has subsequently been seen in the microscope. There is no indication that a best or unique fit of the sequence to the crosslinking data has been found. However, it is encouraging that all twelve assignments involve non-overlapping regions of the sequence which allows their simultaneous construction. The resulting general pattern of secondary structure is very different from previous suggestions for rRNAs or other complex RNAs. It is an RNA chain folded in space much like a typical protein chain. Because of the interwound nature of double helices, base pairing between distant regions of the sequence might result in topologically knotted structures. However, examination of available electron microscopic data suggests that the 16S rRNA does not contain any knots.Keywords
This publication has 18 references indexed in Scilit:
- STRUCTURE AND REACTIONS OF CLOSED DUPLEX DNAAnnual Review of Biophysics and Bioengineering, 1978
- Electron microscopic determination of the binding sites of ribosomal proteins S4 and S8 on 16S RNA.Proceedings of the National Academy of Sciences, 1978
- Structure of the ribosomal 16 S RNA—protein S4 complex as revealed by electron microscopyFEBS Letters, 1977
- An RNA core in the 30S ribosomal subunit of and its structural and functional significanceCell Biology International Reports, 1977
- Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNABiochemistry, 1977
- How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.Proceedings of the National Academy of Sciences, 1975
- Primary sequence of the 16S ribosomal RNA of Escherichia coliNucleic Acids Research, 1975
- Improved Estimation of Secondary Structure in Ribonucleic AcidsNature New Biology, 1973
- The Writhing Number of a Space CurveProceedings of the National Academy of Sciences, 1971
- The twisted circular form of polyoma viral DNA.Proceedings of the National Academy of Sciences, 1965