VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression of vps Biosynthesis Genes and EPS ETr -Associated Phenotypes in Vibrio cholerae O1 El Tor

Abstract

The rugose colonial variant of Vibrio cholerae O1 El Tor produces an exopolysaccharide (EPS^ETr) that enables the organism to form a biofilm and to resist oxidative stress and the bactericidal action of chlorine. Transposon mutagenesis of the rugose variant led to the identification of vpsR, which codes for a homologue of the NtrC subclass of response regulators. Targeted disruption of vpsR in the rugose colony genetic background yielded a nonreverting smooth-colony morphotype that produced no detectable EPS^ETr and did not form an architecturally mature biofilm. Analysis of two genes, vpsA andvpsL, within the vps cluster of EPS^ETr biosynthesis genes revealed that their expression is induced above basal levels in the rugose variant, compared to the smooth colonial variant, and requires vpsR. These results show that VpsR functions as a positive regulator of vpsAand vpsL and thus acts to positively regulate EPS^ETr production and biofilm formation.