Preparation of polydeoxynucleotides linked to a solid support by coupling CNBr-activated cellulose with 5′-NH2-terminated oligo and poly(pdT)'s
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (1), 247-258
- https://doi.org/10.1093/nar/6.1.247
Abstract
Synthetically-prepared 5'-NH2-dT(pdT)n oligomers (66,n=4 or 7) were immobilized on cyanogen bromide activated cellulose. The influence of temperature, pH, and ionic strength on the rate of the coupling process was studied. The oligomer 5'-NH2-DT(pdT)8 could be elongated enzymatically to the polymers 5'-NH2-dT(pdT)n (n=20, 51 and 84), which could be immobilized on cellulose. The cellulose-NH-dT(pdT)84 polymer thus obtained could be assembled to a new solid-state polymer e.g. poly(dA)290 . poly(/3H/dT)200, poly(dT)85-cellulose which, in turn, was a very convenient substrate for assaying DNA-ligase.Keywords
This publication has 17 references indexed in Scilit:
- The activity of mammalian polynucleotide ligase on X-irradiated DNAsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
- Covalent Attachment of DNA to AgaroseEuropean Journal of Biochemistry, 1975
- Studies on polynucleotides. The linkage of deoxyribopolynucleotide templates to cellulose and its use in their replication.1974
- Chemical linkage of nucleic acids to neutral and phosphorylated cellulose powders and isolation of specific sequences by affinity chromatographyBiochemistry, 1974
- Purification of a DNA polymerase from calf thymus nucleiBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- An improved method for the insolubilization of DNA for affinity chromatographyBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Neue Darstellungsmethode von Nucleotid‐Analogen der 5′‐Amino‐5′‐desoxy‐nucleosideEuropean Journal of Inorganic Chemistry, 1972
- Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase.1971
- Covalent attachment of nucleic acids to agarose for affinity chromatographyBiochemistry, 1971
- Polynucleotide Celluloses as Solid State Primers and Templates for PolymerasesJournal of Biological Chemistry, 1968