Effect of macrophage colony-stimulating factor on the function of resident peritoneal macrophages.

Abstract

Resident peritoneal cells of monocyte/macrophage lineage were collected by lavage of mice and incubated in vitro for 1-3d in a culture medium containing various concentrations of macrophage colony-stimulating factor (M-CSF). Stimulation of the cells by zymosan showed that the potency of producing luminol-dependent chemiluminescence had been markedly increased in the CSF-treated cells, indicating increased generation of active oxygen species in these cells. There was an optimal concentration of M-CSF for the enhancement, and the potency of the cells was notably decreased by an overdose of M-CSF. The result was interpreted as being due to the down-regulation of M-CSF receptor.