Optimizing PCR Positivity Criterion for Detection of Herpes Simplex Virus DNA on Skin and Mucosa
- 1 May 2007
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (5), 1618-1620
- https://doi.org/10.1128/jcm.01405-06
Abstract
In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.Keywords
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