Use and evaluation of leucocyte monoclonal antibodies in the diagnostic laboratory: a review
- 1 March 1996
- journal article
- review article
- Published by Wiley in Clinical and Laboratory Haematology
- Vol. 18 (1), 1-5
- https://doi.org/10.1111/j.1365-2257.1996.tb00728.x
Abstract
The diagnosis of haematopoietic disorders by immunological methods, termed 'immunophenotyping', or 'cell marker analysis', is now a routine investigation in many haematology laboratories. A wide range of antibodies and fluorochromes are commercially available, in addition to antigen detection systems: immunocytochemistry, direct or indirect immunofluorescence, incorporating either light microscopy or flow cytometry. In view of this variety in methodology, it is important that all antibodies are used appropriately. The following review highlights a variety of issues, including antibody selection and dilution, negative controls, storage, fixatives as well as quality control, that require consideration prior to the routine use of antibody reagents.Keywords
This publication has 9 references indexed in Scilit:
- Leukaemia immunophenotyping: effect of antibody source and fluorochrome on antigen detection.Journal of Clinical Pathology, 1995
- Immunophenotyping in the diagnosis of chronic lymphoproliferative disorders. General Haematology Task Force of BCSH.Journal of Clinical Pathology, 1994
- Myelomonocytic Antigens are Rarely Expressed on B-Lymphocytic Leukemia CellsLeukemia & Lymphoma, 1993
- The immunologic detection of minimal residual disease in acute leukemia [published erratum appears in Blood 1990 Nov 1;76(7):1901]Blood, 1990
- Biphenotypic leukemic lymphocyte precursors in CD2+CD19+ acute lymphoblastic leukemia and their putative normal counterparts in human fetal hematopoietic tissuesBlood, 1989
- Laboratory control of immunocytochemistryEuropean Journal of Haematology, 1989
- Monocytic differentiation in acute myeloid leukaemia: a cause of Fc binding of monoclonal antibodiesBritish Journal of Haematology, 1986
- Detection of Terminal Deoxynucleotidyl Transferase in Acute Leukemias Using Monoclonal Antibodies Directed against Native and Denatured SitesAmerican Journal of Clinical Pathology, 1986
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975