Novel Screening Procedure for Recombinant Plasmids
- 28 January 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 195 (4276), 391-393
- https://doi.org/10.1126/science.318763
Abstract
Lysed bacterial colonies containing potential recombinant plasmids were mixed with molten agar and sealed into slots of an agarose gel. After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids. Xenopus laevis ribosomal DNA and histone DNA of Psammechinus miliaris were ligated into pCRI plasmids and screened by this method.This publication has 8 references indexed in Scilit:
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