Crystal Structure of Formate Dehydrogenase H: Catalysis Involving Mo, Molybdopterin, Selenocysteine, and an Fe 4 S 4 Cluster
- 28 February 1997
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 275 (5304), 1305-1308
- https://doi.org/10.1126/science.275.5304.1305
Abstract
Formate dehydrogenase H from Escherichia coli contains selenocysteine (SeCys), molybdenum, two molybdopterin guanine dinucleotide (MGD) cofactors, and an Fe4S4 cluster at the active site and catalyzes the two-electron oxidation of formate to carbon dioxide. The crystal structures of the oxidized [Mo(VI), Fe4S4(ox)] form of formate dehydrogenase H (with and without bound inhibitor) and the reduced [Mo(IV), Fe4S4(red)] form have been determined, revealing a four-domain αβ structure with the molybdenum directly coordinated to selenium and both MGD cofactors. These structures suggest a reaction mechanism that directly involves SeCys140 and His141 in proton abstraction and the molybdenum, molybdopterin, Lys44, and the Fe4S4 cluster in electron transfer.Keywords
This publication has 18 references indexed in Scilit:
- Crystal Structure of DMSO Reductase: Redox-Linked Changes in Molybdopterin CoordinationScience, 1996
- Characterization of Crystalline Formate Dehydrogenase H from Escherichia coliPublished by Elsevier ,1996
- Modeling the Tungsten Sites of Inactive and Active Forms of Hyperthermophilic Pyrococcus furiosus Aldehyde Ferredoxin OxidoreductaseJournal of the American Chemical Society, 1996
- NAD-binding domains of dehydrogenasesCurrent Opinion in Structural Biology, 1995
- Crystal Structure of the Xanthine Oxidase-Related Aldehyde Oxido-Reductase from D. gigasScience, 1995
- SETOR: Hardware-lighted three-dimensional solid model representations of macromoleculesJournal of Molecular Graphics, 1993
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991
- Improved methods for building protein models in electron density maps and the location of errors in these modelsActa Crystallographica Section A Foundations of Crystallography, 1991
- A fast algorithm for rendering space-filling molecule picturesJournal of Molecular Graphics, 1988
- Inactivation of formate dehydrogenase from Methanobacterium formicicum by cyanideBiochemistry, 1986