INHIBITION OF SYNTHESIS OF POLYAMINES AND DNA IN ACTIVATED LYMPHOCYTES BY A COMBINATION OF ALPHA-METHYLORNITHINE AND METHYLGLYOXAL BIS(GUANYLHYDRAZONE)

Abstract

The cancer chemotherapeutic drug, methylglyoxal bis(guanylhydrazone), inhibits the synthesis of spermidine and spermine, but allows continued putrescine production in small [bovine] lymphocytes stimulated by concanavalin A. DNA replication in these cells is inhibited 50% while the synthesis of protein and RNA continues normally. When excess putrescine accumulation in the presence of methylglyoxal bis(guanylhydrazone) was inhibited with .alpha.-methylornithine, a competitive inhibitor of ornithine decarboxylase, the inhibition of DNA replication was accentuated, with still no effect on protein or RNA synthesis. No inhibition of DNA synthesis by the combination of .alpha.-methylornithine and methylglyoxal bis(guanylhydrazone) was observed when the inhibitors were added after accumulation of cellular polyamines. Inhibition was reversed by exogenous putrescine, spermidine or spermine. Putrescine can partly fulfill the role normally played by spermidine and spermine in DNA replication, and blocking putrescine synthesis in the presence of methylglyoxal bis(guanylhydrazone) amplifies the polyamine requirement. The implications of this with regard to polyamine synthesis as a site of chemotherapy are discussed.