Melting and chemical modification of a cyclized self-splicing group I intron: similarity of structures in 1 M sodium, in 10 mM magnesium and in the presence of substrate
- 1 November 1990
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 29 (44), 10147-10158
- https://doi.org/10.1021/bi00496a002
Abstract
C IVS is the cyclized form of the intron from the RNA precursor of the Tetrahymena thermophila large subunit (LSU) ribosomal RNA. C IVS was mapped by chemical modification in 1 M Na+, 0.05 M Na+ and 10 mM Mg2+ (Na+/Mg2+), and Na+/Mg2+ with CUCU substrate. The results suggest the secondary structure is similar for all three conditions. Optical melting curves were also measured for C IVS in 1 M Na+ and Na+/Mg2+ and indicate the secondary structures have similar stabilities under both conditions. Computer predictions of secondary structure and stability are in good agreement with observations. The results suggest that many of the approximations used for computer prediction of secondary structure by free energy minimization are reasonable.This publication has 40 references indexed in Scilit:
- Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: structural homology with fungal mitochondrial intervening sequences.Proceedings of the National Academy of Sciences, 1983
- Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reactionNature, 1983
- Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.The EMBO Journal, 1983
- Making ends meet: a model for RNA splicing in fungal mitochondriaNature, 1982
- Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymenaCell, 1982
- Synthesis of deoxyoligonucleotides on a polymer supportJournal of the American Chemical Society, 1981
- Structure and structure formation of viroidsJournal of Molecular Biology, 1979
- Direct chemical method for sequencing RNA.Proceedings of the National Academy of Sciences, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977