Polypeptide-chain stoicheiometry and lipoic acid content of the pyruvate dehydrogenase complex of Escherichia coli
- 1 January 1979
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 177 (1), 129-136
- https://doi.org/10.1042/bj1770129
Abstract
The pyruvate dehydrogenase multienzyme complex was isolated from E. coli grown in the presence of [35S]sulfate. The 3 component enzymes were separated by sodium dodecyl sulfate/ polyacrylamide-gel electrophoresis, and the molar ratios of the 3 polypeptide chains were determined by measurement of the radioactivity in each band. The chain ratio of lipoamide dehydrogenase to lipoate acetyltransferase approached unity, but there was a molar excess of chains of the pyruvate decarboxylase component. The 35S-labeled complex was also used in a new determination of the total lipoic acid content. Each polypeptide chain of the lipoate acetyltransferase component appears to bear at least 3 lipoyl groups.This publication has 31 references indexed in Scilit:
- Fluorescence energy transfer measurements in the pyruvate dehydrogenase multienzyme complex from Escherichia coli with chemically modified lipoic acidBiochemistry, 1977
- Acetylation stoichiometry of Escherichia coli pyruvate dehydrogenase complexBiochemical and Biophysical Research Communications, 1977
- Polypeptide chain stoicheiometry in the self‐assembly of the pyruvate dehydrogenase multienzyme complex of Escherichia coliFEBS Letters, 1977
- Redetermination of the molecular weights of the components of the pyruvate dehydrogenase complex from E. coli K12Biochemical and Biophysical Research Communications, 1977
- Use of dimethyl suberimidate and novel periodate-cleavable bis(imido esters) to study the quaternary structure of the pyruvate dehydrogenase multienzyme complex of Escherichia coliBiochemistry, 1976
- The stoichiometry of polypeptide chains in the pyruvate dehydrogenase multienzyme complex of E. Coli determined by a simple novel methodFEBS Letters, 1975
- X-ray small-angle studies of the pyruvate dehydrogenase core complex from Escherichia coli K-12European Biophysics Journal, 1975
- The Subunit Structure of the Escherichia coli K12 Pyruvate‐Dehydrogenase ComplexEuropean Journal of Biochemistry, 1973
- The subunit molecular weights of the α‐ketoacid dehydrogenase multienzyme complexes from E. coliFEBS Letters, 1971
- Pyruvate Dehyrogenase Component Subunit Structure of the Escherichia coli K 12 Pyruvate Dehydrogenase ComplexEuropean Journal of Biochemistry, 1971