The Aminoacyladenylate Mechanism in the Aminoacylation Reaction of Yeast Phenylalanyl-tRNA Synthetase
- 1 April 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 85 (1), 85-88
- https://doi.org/10.1111/j.1432-1033.1978.tb12214.x
Abstract
It is shown from a combination of rapid quenching and steady-state kinetics that the phenylalanyl-tRNA synthetase from yeast catalyzes the formation of phenylalanyl-tRNA by the aminoacyladenylate pathway at pH 7.8 and 25.degree. C. The rate-determining step at saturating reagent concentrations is not the dissociation of the charged tRNA from the enzyme.This publication has 10 references indexed in Scilit:
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