Fluorescent False Neurotransmitters Visualize Dopamine Release from Individual Presynaptic Terminals
- 12 June 2009
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 324 (5933), 1441-1444
- https://doi.org/10.1126/science.1172278
Abstract
The nervous system transmits signals between neurons via neurotransmitter release during synaptic vesicle fusion. In order to observe neurotransmitter uptake and release from individual presynaptic terminals directly, we designed fluorescent false neurotransmitters as substrates for the synaptic vesicle monoamine transporter. Using these probes to image dopamine release in the striatum, we made several observations pertinent to synaptic plasticity. We found that the fraction of synaptic vesicles releasing neurotransmitter per stimulus was dependent on the stimulus frequency. A kinetically distinct “reserve” synaptic vesicle population was not observed under these experimental conditions. A frequency-dependent heterogeneity of presynaptic terminals was revealed that was dependent in part on D2 dopamine receptors, indicating a mechanism for frequency-dependent coding of presynaptic selection.Keywords
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