Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy

Abstract
The temporal correlations of thermodynamic concentration fluctuations have been measured in a chemically reactive system at equilibrium by observing fluctuations of the fluorescence of a reaction product. The experiment yields the chemical rate constants and diffusion coefficients and shows the coupling among them. Data are reported for binding of ethidium bromide to DNA.