CANCER-THERAPY WITH CHEMICALLY MODIFIED ENZYMES .1. ANTITUMOR PROPERTIES OF POLYETHYLENE GLYCOL-ASPARAGINASE CONJUGATES
- 1 January 1984
- journal article
- research article
- Vol. 7 (2), 175-186
Abstract
The covalent attachment of monomethoxypolyethylene glycol (PEG) to asparaginases from Escherichia coli and Vibrio succinogenes by new coupling methodology produced conjugates that are active, stable, without significant immune response and with greatly extended plasma half-lives in mice. Therapeutic efficacies were greater for the PEG-asparaginases than for the unmodified asparaginases in mice infected with the rat L5178Y lymphosarcoma or the mouse 6C3HED tumor. Large single doses of native or modified enzymes were more effective against tumors than the same amount of enzyme given in smaller doses over several days.This publication has 9 references indexed in Scilit:
- IMMUNOLOGICAL EFFECTS OF NATIVE AND POLYETHYLENE GLYCOL-MODIFIED ASPARAGINASES FROM VIBRIO-SUCCINOGENES AND ESCHERICHIA-COLI IN NORMAL AND TUMOR-BEARING MICE1982
- HYPOURICAEMIC EFFECT OF POLYETHYLENEGLYCOL MODIFIED URATE OXIDASEThe Lancet, 1981
- PHARMACOLOGY OF ESCHERICHIA-COLI-L-ASPARAGINASE POLYETHYLENE-GLYCOL ADDUCT1981
- REDUCTION OF PLASMA URATE LEVELS IN THE COCKEREL WITH POLYETHYLENE GLYCOL-URICASE1981
- IMMUNOSUPPRESSIVE PROPERTIES AND CIRCULATING LIFE OF ACHROMOBACTER GLUTAMINASE-ASPARAGINASE COVALENTLY ATTACHED TO POLYETHYLENE-GLYCOL IN MAN1981
- COMPARISON OF THE IMMUNOSUPPRESSIVE EFFECTS OF ASPARAGINASES FROM ESCHERICHIA-COLI AND VIBRIO-SUCCINOGENES1980
- Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase.Journal of Biological Chemistry, 1977
- Purification and characterization of L-asparaginase with anti-lymphoma activity from Vibrio succinogenes.Journal of Biological Chemistry, 1976
- Determination of free amino groups in proteins by trinitrobenzenesulfonic acidAnalytical Biochemistry, 1966