Hydrolyses of 1-O-Alkyl-, 1-O-Alkenyl-, and 1-Acyl-2-[1-14C]-linoleoyl-glycero-3-phosphorylcholine by Various Phospholipases

Abstract

1. 1-O-Alkyl-, 1-O-alkenyl- and 1-acyl-2-[l-¹⁴C]-linoleoyl-glycero-3-phosphorylcholine were synthesized enzymatically and submitted to hydrolyses by various phospholipases. 2. Among the phospholipases A [EC 3.1.1. 4] of snake venoms, those of species of Crotalus mainly hydrolyzed diacyl compound and those of species of Naja naja liydrolyzed diacyl and 1-O-alkyl-2-acyl compounds at the same rates and 1-O-alkenyl-2-acyl compound at half this rate. 3. Phospholipase C [EC 3. 1. 4. 3] of Cl. perfringens was specific for diacyl compound while that of B. cereus hydrolyzed 1-O-alkyl-2-acyl compound three times faster than diacyl compound. 4. Cabbage and carrot phospholipases D [EC 3. 1. 4. 4] hydrolyzed 1-O-alkyl-2-acyl compound at one third the rate of diacyl compound and 1-O-alkenyl-2-acyl compound at 10% of this rate.