Disulfide engineering at the dimer interface of lactobacillus casei thymidylate synthase: Crystal structure of the T155C/E188C/C244T mutant
- 1 January 1999
- journal article
- Published by Wiley in Protein Science
- Vol. 8 (4), 930-933
- https://doi.org/10.1110/ps.8.4.930
Abstract
The crystal structure of a covalently cross-linked Lactobacillus casei thymidylate synthase has been determined at 2.8 A resolution. The sites for mutation to achieve the bis-disulfide linked dimer were identified using the disulfide modeling program MODIP. The mutant so obtained was found to be remarkably thermostable. This increase in stability has been reasoned to be entirely a consequence of the covalent gluing between the two subunits.Keywords
This publication has 18 references indexed in Scilit:
- Cavity-Creating Mutation at the Dimer Interface of Plasmodium falciparum Triosephosphate Isomerase: Restoration of Stability by Disulfide Cross-Linking of SubunitsBiochemistry, 1998
- The entropy cost of protein associationJournal of Molecular Biology, 1997
- Covalent tethering of the dimer interface annuls aggregation in thymidylate synthaseProtein Science, 1996
- Covalent Reinforcement of a Fragile Region in the Dimeric Enzyme Thymidylate Synthase Stabilizes the Protein against Chaotrope-Induced UnfoldingBiochemistry, 1996
- Thermal stabilization of thymidylate synthase by engineering two disulfide bridges across the dimer interfaceJournal of Molecular Biology, 1994
- Intersubunit disulfide-bonded λ-Cro proteinProtein Engineering, Design and Selection, 1991
- Improved methods for building protein models in electron density maps and the location of errors in these modelsActa Crystallographica Section A Foundations of Crystallography, 1991
- Stereochemical modeling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesisProtein Engineering, Design and Selection, 1989
- Engineering of an intersubunit disulphide bridge in glutathione reductase from Escherichia coliFEBS Letters, 1988
- The use of an imaging proportional counter in macromolecular crystallographyJournal of Applied Crystallography, 1987