Direct Electrochemistry of Cytochrome c at a Glassy Carbon Electrode Modified with Single-Wall Carbon Nanotubes

Abstract
The electrochemistry of horse heart cytochrome c was studied by cyclic voltammetry at a glassy carbon electrode modified with single-wall carbon nanotubes (SWNTs). A pair of well-defined redox waves was obtained in cytochrome c aqueous solution at an activated SWNT film-modified electrode. The optimal conditions for activating the SWNT film-modified electrode has been determined. The electrode reaction of cytochrome c is a diffusion-controlled process. The peak current increases linearly with the concentration of cytochrome c in the range from 3.0 × 10-5−7.0 × 10-4 M. The detection limit is 1.0 × 10-5 M. The activated SWNT film was characterized by scanning electron microscopy. Furthermore, interaction of cytochrome c with adenine was characterized by electrochemical and spectral methods.