The annelid phosphokinases

Abstract

Guanidine phosphokinases were extracted from annelid muscle in 2 volumes of ice cold water. The extract was allowed to stand at 0[degree]C for 10 mins. and then 5 volumes of cold acetone was added with stirring. The extract was filtered and the acetone dried powder contained the enzymes. Transphosphorylation was demonstrated and taurocyamine phosphate, glycocyamine phosphate, arginine phosphate, and creatine phosphate were identified. It was concluded that taurocyamine phosphate and glycocyamine phosphate as well as creatine phosphate can act as phosphagens in certain of the annelids.