THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZE
Open Access
- 1 April 1974
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 139 (4), 1031-1036
- https://doi.org/10.1084/jem.139.4.1031
Abstract
Membrane-associated immunoglobulin (M-Ig) was isolated from Daudi cells which had been radioiodinated using lactoperoxidase. This M-Ig was found to have a molecular size on SDS-PAGE of 330,000 daltons. The component protein chains (µ and K) have molecular sizes of 75,000 and 33,000 daltons, respectively. Further studies showed that [3H]galactose can be incorporated into K-chains, but that extra protein and not carbohydrate was responsible for the increased molecular size of this molecule.Keywords
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