Vacuolar H+-ATPase: From mammals to yeast and back
- 1 December 1996
- journal article
- review article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 52 (12), 1101-1110
- https://doi.org/10.1007/bf01952108
Abstract
Vacuolar H+-adenosine triphosphatase (V-ATPase) is composed of distinct catalytic (V1) and membrane (V0) sectors containing several subunits. The biochemistry of the enzyme was mainly studied in organelles from mammalian cells such as chromaffin granules and clathrin-coated vesicles. Subsequently, mammalian cDNAs and yeast genes encoding subunits of V-ATPase were cloned and sequenced. The sequence information revealed the relation between V- and F-ATPases that evolved from a common ancestor. The isolation of yeast genes encoding subunits of V-ATPase opened an avenue for molecular biology studies of the enzyme. Because V-ATPase is present in every known eukaryotic cell and provides energy for vital transport systems, it was anticipated that disruption of genes encoding V-ATPase subunits would be lethal. Fortunately, yeast cells can survive the absence of V-ATPase by ‘drinking’ the acidic medium. So far only yeast cells have been shown to be viable without an active V-ATPase. In contrast to yeast, mammalian cells may have more than one gene encoding each of the subunits of the enzyme. Some of these genes encode tissue- and/or organelle-specific subunits. Expression of these specific cDNAs in yeast cells may reveal their unique functions in mammalian cells. Following the route from mammals to yeast and back may prove useful in the study of many other complicated processes.Keywords
This publication has 103 references indexed in Scilit:
- Resolution of Subunit Interactions and Cytoplasmic Subcomplexes of the Yeast Vacuolar Proton-translocating ATPasePublished by Elsevier ,1996
- Common Principles of Protein Translocation Across MembranesScience, 1996
- Hairpin folding of subunit c of F1Fo ATP synthase: 1H distance measurements to nitroxide-derivatized aspartyl-61Biochemistry, 1994
- Saccharomyces cerevisiae expression of exogenous vacuolar ATPase subunits BBiochimica et Biophysica Acta (BBA) - Biomembranes, 1993
- Sensitivity to nitrate and other oxyanions further distinguishes the vanadate-sensitive osteoclast proton pump from other vacuolar hydrogen ion-ATPasesBiochemistry, 1993
- Structure, Function, and Mutational Analysis of V‐ATPasesAnnals of the New York Academy of Sciences, 1992
- Identification of the subunits of F1F0-ATPase from bovine heart mitochondriaBiochemistry, 1991
- The progenitor of ATP synthases was closely related to the current vacuolar H+‐ATPaseFEBS Letters, 1989
- Cellular Biology and Biochemical Mechanism of Bone ResorptionClinical Orthopaedics and Related Research, 1988
- H+-ATPases from mitochondria, plasma membranes, and vacuoles of fungal cellsThe Journal of Membrane Biology, 1986