Influence of a preadsorbed terpolymer on human platelet accumulation, fibrinogen adsorption, and ex vivo blood activation in hemodialysis hollow fibers
- 1 January 1992
- journal article
- Published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition
- Vol. 3 (5), 389-402
- https://doi.org/10.1163/156856292x00204
Abstract
Results are presented on kinetics of platelet accumulation in charged polyacrylonitrile (AN69) hollow fibers by continuous data recording under flow conditions (wall shear rate 108-1050 s-1), using suspensions of washed 111In-labeled human platelets in Tyrode's-albumin buffer, containing washed red blood cells (0-40%). Preadsorption of a terpolymer of acrylonitrile, poly(ethyleneoxide) methacrylate and trimethylaminoethyl chloride methacrylate leads to very efficient passivation with respect to platelet accumulation and fibrinogen adsorption. In human ex vivo tests, evaluation of complement peptide C3a, platelet βthromboglobulin, leucocyte-polymorphonuclear neutrophile elastase and fibrinopeptide A shows no detectable activation. Furthermore, preadsorption appears to result in simultaneous improvement in hemocompatibility of the blood lines leading to and from the dialysis module. This single pretreatment of dialysis membranes should allow injection of lower doses of anticoagulant to patients submitted to hemodialysis.Keywords
This publication has 28 references indexed in Scilit:
- Physicochemical and biochemical aspects of fibrinogen adsorption from plasma and binary protein solutions onto polyethylene and glassJournal of Colloid and Interface Science, 1988
- Protein adsorption on polymeric biomaterials I. Adsorption isothermsJournal of Colloid and Interface Science, 1988
- Adsorption of human albumin and fibrinogen onto heparin-like materials I. Adsorption isothermsColloids and Surfaces, 1985
- Adsorption of human albumin and fibrinogen onto heparin-like materials II. Desorption and exchange processesColloids and Surfaces, 1985
- Adsorption kinetics of plasma proteins onto silicaColloids and Surfaces, 1984
- Interaction of fibrinogen with solid surfaces of varying charge and hydrophobic—hydrophilic balanceJournal of Colloid and Interface Science, 1983
- Interaction of fibrinogen with solid surfaces of varying charge and hydrophobic—hydrophilic balanceJournal of Colloid and Interface Science, 1983
- Protein adsorption on crosslinked polydimethylsiloxane using total internal reflection fluorescenceJournal of Colloid and Interface Science, 1983
- Total internal reflection fluorescence: a technique for examining interactions of macromolecules with solid surfacesJournal of Colloid and Interface Science, 1983
- Dependence of albumin–fibrinogen simple and competitive adsorption on surface properties of biomaterialsJournal of Polymer Science: Polymer Symposia, 1979