Improved Methods for Preparing Pure Gibberellins from Cultures of Gibberella fujikuroi. Isolation by Adsorption or Partition Chromatography on Silicic Acid and by Partition Chromatography on Sephadex Columns

Abstract

Procedures have been developed for the separation of closely related gibberellins on a preparative scale by partition chromatography on columns of Sephadex. This technique simplifies the isolation of gibberellins which have previously been difficult to obtain, or have been overlooked. It has particular value in preparing radiochemically pure gibberellins after biosynthetic labeling. Selective fermentations were used to promote the formation of required gibberellins by Gibberella fujikuroi. These gave enriched mixtures which, after an initial fractionation by silicic acid chromatography, were conveniently separated by Sephadex partition chromatography to yield pure compounds.