Isolation and characterization of vitamin K-dependent region of bovine blood clotting factor X.
Open Access
- 1 April 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (4), 1281-1285
- https://doi.org/10.1073/pnas.72.4.1281
Abstract
A 39-residue peptide from the tryptic digestion of bovine blood clotting factor X has been isolated by specific adsorption on barium citrate. The amino- and carboxyl-terminal sequences of the peptide were determined and compared to the vitamin K-dependent Ca2+-binding region from bovine prothrombin. The factor X peptide was found to contain gamma-carboxyglutamic acid residues, and the results of independent analysis are consistent with all 14 glutamic acid residues as gamma-carboxyglutamic acid. The similarity of the factor X peptide to the prothrombin peptide supports the hypothesis that the vitamin K-dependent blood clotting proteins are descended from a common ancestral gene.Keywords
This publication has 24 references indexed in Scilit:
- Vitamin K Dependent Modifications of Glutamic Acid Residues in ProthrombinProceedings of the National Academy of Sciences, 1974
- Vitamin K and the biosynthesis of prothrombin. 3. Structural comparison of an NH2-terminal fragment from normal and from dicoumarol-induced bovine prothrombin.1973
- Mode of action of vitamin K. Calcium binding properties of bovine prothrombinBiochemistry, 1972
- Bovine factor X1 (Stuart factor). Mechanism of activation by a protein from Russell's viper venomBiochemistry, 1972
- Bovine factor X1a (activated Stuart factor). Evidence of homology with mammalian serine proteasesBiochemistry, 1972
- The carbohydrate of bovine prothrombin. Partial structural determination demonstrating the presence of -galactose residues.1972
- A Study of the Reaction Product of Factor VIII and Factor IX by Gel FiltrationThrombosis and Haemostasis, 1967
- The Activation of Antihemophilic Factor (Factor VIII) by Activated Christmas Factor (Activated Factor IX)*Biochemistry, 1964
- Observations on the interaction of phospholipids and certain clotting factors in prothrombin activator formationBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- The Stuart-Prower Factor Assay and its Clinical SignificanceThrombosis and Haemostasis, 1958