Purification of the araC Protein
- 1 September 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (9), 2145-2148
- https://doi.org/10.1073/pnas.68.9.2145
Abstract
The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-beta-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.Keywords
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