Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures.

  • 1 September 1972
    • journal article
    • Vol. 24 (3), 488-94
Abstract
Murine ribonucleic acid tumor viruses and C-type virus particles are produced in relatively large quantities in roller bottle cultures. The viruses present in large volumes of culture fluids can be purified by a simple two-step procedure involving polyethylene glycol precipitation and equilibrium centrifugation in sucrose density gradients.