CELLS CULTURED FROM THE DIABETIC (DB/DB) MOUSE HAVE A PERMANENT DECREASE IN INSULIN RECEPTORS*
- 1 November 1980
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 107 (5), 1652-1655
- https://doi.org/10.1210/endo-107-5-1652
Abstract
Investigation of mechanisms responsible for the decreased numbers of insulin receptors observed in obesity and diabetes has been facilitated by the development of cell culture systems permitting study of cellular events independent of fluctuating hormone levels and multiple endocrine interactions present in the whole organism. With such a system, we have found that cells cultured from the skin of diabetic mice have 45–48% fewer receptors for insulin than those from nondiabetic littermates. This difference is maintained in culture over many generations, suggesting that the decreased expression of insulin receptors in these cells is related to the genetic trait for diabetes.Keywords
This publication has 5 references indexed in Scilit:
- Diabetes and Cell Growth—Lack of Differences in Growth Characteristics of Fibroblasts from Diabetic and Nondiabetic Pima IndiansDiabetes, 1980
- Insulin receptors and the site of action of insulinLife Sciences, 1978
- Characterization of the binding of multiplication-stimulating activity to a receptor for growth polypeptides in chick embryo fibroblasts.Journal of Biological Chemistry, 1977
- Mitogen receptors in chick embryo fibroblasts. Kinetics, specificity, unmasking, and synthesis of 125I-insulin binding sites.Journal of Biological Chemistry, 1976
- Insulin Receptors in Human and Animal Placental TissueDiabetes, 1974