KINETICS OF NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENATION BY HUMAN PLACENTA
- 1 April 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 95 (1), 150-155
- https://doi.org/10.1042/bj0950150
Abstract
Measurements of kinetic constants for a purified preparation of human-placenta estradiol dehydrogenase have been made. These constants have been compared with similar measurements made on crude ammonium sulphate precipitates of human-placenta homogenates. The comparison indicates that nearly half of the observed nicotinamide nucleotide-transhydrogenation activity in the crude preparations is due to a specific estrogen-dependent transhydrogenase. The remainder of the observed activity results from a substrate-mediate transhydrogenation catalysed by the estradiol-dehydrogenase activities present in the preparation.Keywords
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