Intracellular Interaction and Metabolic Fate of Arsenite in the Rabbit

Abstract

Rabbits were treated with single doses of 50 micrograms AsO2-/kg b.w. by IP injection. At 48 h, 83.0 +/- 2.1% of arsenic is excreted via the urine. Also in blood the clearance of arsenic is rapid. Liver, kidney, and lung, among the tissues tested, show the highest concentration of arsenic at 5, 16, and 48 h after injection. Arsenic was found to be present at subcellular levels mainly in the nuclear and soluble fractions of the above-mentioned tissues. Chromatographic studies performed by gel filtration have shown that in plasma and in lung cytosol, arsenic is present, associated mainly to low molecular weight components, whereas in liver and kidney cytosol it is prevalently recovered in the fractions corresponding to the high molecular weight components. This different interaction of arsenic with molecular components was shown to influence the metabolic fate of this compound in the rabbit.