Murine embryonal carcinoma hybrids: Decreased ability to spontaneously differentiate as a dominant trait
- 1 November 1981
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 109 (2), 195-204
- https://doi.org/10.1002/jcp.1041090202
Abstract
The F9 murine embryonal carcinoma (ec) cell line does not differentiate spontaneously at high frequency either in vitro or in vivo. A derivative of this variant ec cell line resistant to ouabain and thioguanine was fused with the PSA1 ec cell line, which retains the ability to differentiate to a variety of cell types, either in vitro or in vivo. Hybrids were selected in media containing ouabain, hypoxanthine, aminopterin, and thymidine at a frequency of approximately 10−3. Authentic hybrids were chosen by screening for the expected hybrid DNA content by flow microfluorometry and the lack of parental cell contamination in appropriate selective media. After injection into strain 129 mice, five of eight independently isolated hybrids formed tumors composed exclusively of embryonal carcinoma cells. The remaining three hybrids of this cross caused tumors composed of greater than 95% embryonal carcinoma cells but with small foci of differentiated tissues as well. Like the F9‐derived parental line, less than 10 percent of the clones, formed by the hybrids after six days of growth, contained cells that secreted plasminogen activator. Since control hybrids derived from the fusion of either two F9 derivatives or two pluripotent cell lines resembled their parental cell lines with regard to the types of tumors formed and their differentiation in vitro, it appears that the F9 phenotype is dominant to the pluripotent phenotype in early passage embryonal carcinoma hybrids.This publication has 29 references indexed in Scilit:
- Studies on the effect of retinoids on the differentiation of teratocarcinoma stem cells in vitro and in vivoDevelopmental Biology, 1980
- PCC4azal teratocarcinoma stem cell differentiation in culture: III. Cell-to-cell communication propertiesDevelopmental Biology, 1980
- Stimulation of differentiation of several murine embryonal carcinoma cell lines by retinoic acidExperimental Cell Research, 1979
- Effect of intercellular communication on the selection of intraspecific human hybrids in HAT and ouabainSomatic Cell and Molecular Genetics, 1978
- Mosaic mice with teratocarcinoma-derived mutant cells deficient in hypoxanthine phosphoribosyltransferaseProceedings of the National Academy of Sciences, 1977
- Metabolic co-operation in HGPRT+ and HGPRT− embryonal carcinoma cellsDevelopmental Biology, 1977
- Teratocarcinoma differentiation: Plasminogen activator activity associated with embryoid body formationCell, 1977
- Alkaline Phosphatase Activity in Mouse TeratomaProceedings of the National Academy of Sciences, 1973
- RETENTION OF MULTIPLE DEVELOPMENTAL POTENTIALITIES BY CELLS OF A MOUSE TESTICULAR TERATOCARCINOMA DURING PROLONGED CULTURE in vitro AND THEIR EXTINCTION UPON HYBRIDIZATION WITH CELLS OF PERMANENT LINESProceedings of the National Academy of Sciences, 1967
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964