PHARMACOLOGICAL EVIDENCE FOR THE EXISTENCE OF TWO COMPONENTS IN THE TWITCH RESPONSE TO FIELD STIMULATION OF DETRUSOR STRIPS FROM THE RAT URINARY BLADDER

Abstract

Isolated strips from the anterior dome of the rat urinary bladder respond to single pulse field stimulation with a contraction. Two distinct components of this contraction (''early'' and ''late'') could be observed, both of which were unaffected by hexamethonium (10 .mu.M) and almost abolished by tetrodotoxin (1 .mu.M) indicating their dependence on neurotransmitter release from postganglionic nerve endings. Atropine (3 .mu.M) inhibited the ''late'' component (over 60%) to a significantly greater extent than the ''early'' component (less than 10%) Amplitude of the ''early'' component was usually greater than that of the ''late'' component. There was almost no difference between the ''early'' and ''late'' component in respect of their relationship to stimulus strength and pulse duration. Physostigmine (0.03 .mu.M) enhanced both components of the nerve-mediated contraction, although enhancement of the ''late'' component was much greater than that of the ''early'' one. Tetraethylammonium (TEA, 0.5-5 mM) enhanced, in a concentration-related manner, both ''early'' and ''late'' components of the nerve-mediated contraction. Following exposure to physostigmine or TEA (5 mM) both ''early'' and ''late'' components of contraction were almost completely inhibited by tetrodotoxin. Atropine inhibition was more evident on the ''late'' as compared to ''early'' component of contraction. These findings demonstrate the presence, in the twitch response of rat isolated urinary bladder to field stimulation, of two nerve-mediated components which exhibit a different susceptibility to atropine and physostigmine.