ANTIBODY-MEDIATED SUPPRESSION OF TUMOR-GROWTH .3. MOLECULAR ASSAY OF MURINE IGG1 ALLOANTIBODY REQUIRED TO CAUSE TUMOR SUPPRESSION INVIVO

Abstract

A radioimmunoassay was used to quantitate the number of [mouse lymphoma 6C3HED] tumor cell-bound IgG1 [immunoglobulin G1] anti-tumor antibody molecules required to cause suppression of tumor growth in C3H mice. Radiolabeled anti-IgG1 was used to detect cell-bound IgG1 antibody. The assay was calibrated by using TNP[trinitrophenol]-coupled tumor cells that had a known number of bound IgG1 anti-DNP [dinitrophenol] molecules. From 70,000-130,000 IgG1 anti-tumor antibody molecules per tumor cell were sufficient to cause 50% suppression of tumor growth in mice inoculated with approximately 50,000 tumor cells.