Attenuation of coronary autoregulation in the isolated rabbit heart by endothelium derived nitric oxide

Abstract

Objective: The aim was to investigate the role of endothelium derived nitric oxide (EDNO/EDRF) in the control of coronary autoregulation. Methods: In isolated saline perfused rabbit hearts coronary flow responses to stepwise increases in perfusion pressure were studied under control conditions, during maximum dilatation with sodium nitroprusside, and in the presence of the inhibitor of EDNO synthesis, N^G-nitro-L-arginine (L-NNA), or the vasoconstrictors endothelin-1 and arginine vasopressin. Results: At a constant perfusion pressure of 60 mm Hg. infusion of L-NNA (30 μM), but not D-NNA, reduced the coronary flow from 24.7(SEM 2) to 13.6(2.2) ml·min⁻¹ and abolished flow increases induced by the EDRF stimulator acetylcholine. Under these conditions, pressure induced coronary flow increases were reduced (p−1. These changes occurred in spite of a decrease in the myocardial oxygen uptake from 5.1(0.6) to 3.4(0.5) ml·100 g⁻¹·min⁻¹ (p−1 (pConclusions: EDNO attenuates coronary autoregulatory responses which, if unopposed, potentially impair a functionally adequate myocardial perfusion. It is suggested that the modulator role of EDNO is. at least in part, specific and most likely to be due to shear dependent alterations of EDNO release. Cardiovascular Research 1994;28:414-419