Mapping Interfacial Chemistry Induced Variations in Protein Adsorption with Scanning Force Microscopy
- 2 May 2000
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 72 (11), 2627-2634
- https://doi.org/10.1021/ac991137e
Abstract
In this work, we demonstrate the sensitivity of scanning force microscopy (SFM), operated in friction force mode, to adsorbed protein conformation or orientation. We employ patterned films of methyl- and carboxylate-terminated alkanethiolate monolayers on gold as substrates for protein adsorption to observe the effect of each functional group in the same image. Infrared spectroscopic and SFM studies of bovine fibrinogen (BFG) adsorption to single-component monolayers indicate that complete films of BFG that are stable to imaging are formed at each functional group. After adsorption of BFG to a patterned monolayer, we observe a contrast in friction images due to differences in adsorbed BFG conformation or orientation induced by each functional group. We also observe frictional contrast in films of other proteins adsorbed on patterned monolayers. These observations lead to the conclusion that SFM-measured friction is sensitive to adsorbed protein state.Keywords
This publication has 32 references indexed in Scilit:
- Chemical and Biochemical Analysis Using Scanning Force MicroscopyChemical Reviews, 1999
- Pleated Sheets and Turns ofβ-Peptides with Proteinogenic Side ChainsAngewandte Chemie International Edition, 1999
- Human Growth Hormone Adsorption Kinetics and Conformation on Self-Assembled MonolayersLangmuir, 1998
- Acid−Base Properties and the Chemical Imaging of Surface-Bound Functional Groups Studied with Scanning Force MicroscopyThe Journal of Physical Chemistry B, 1997
- Surface plasmon resonance for real time in situ analysis of protein adsorption to polymer surfacesBiomaterials, 1997
- Imaging Globular and Filamentous Proteins in Physiological Buffer Solutions with Tapping Mode Atomic Force MicroscopyLangmuir, 1995
- Bovine serum albumin conformation on methyl and amine functionalized surfaces compared by scanning force microscopyJournal of Biomedical Materials Research, 1995
- Wetting and Protein Adsorption on Self-Assembled Monolayers of Alkanethiolates Supported on Transparent Films of GoldJournal of the American Chemical Society, 1994
- Fourier Transform Infrared Spectroscopic and Attenuated Total Reflectance Studies of Protein Adsorption in Flowing SystemsPublished by American Chemical Society (ACS) ,1987
- Adsorption of Fibronectin to Polyurethane Surfaces: Fourier Transform Infrared Spectroscopic StudiesPublished by American Chemical Society (ACS) ,1987