Separation of the Spectral Radiance Factor Curve of Fluorescent Substances into Reflected and Fluoresced Components

Abstract

When a spectrophotometer with the correct optical configuration is used to measure a fluorescent sample, the resulting spectral radiance factor curve consists of the sum of a reflected and a fluoresced portion. The true reflectance curve can be obtained by direct measurement below the emission region and also above the absorption region by the use of a fluorescence-killing filter. Between these two limits, in the region of simultaneous absorption and emission (the crossover region), the true reflectance curve can be obtained by calculation from two different spectral radiance factor curves and a single reading through the fluorescence-killing filter.