Gel filtration analysis of vanadium inAscidia nigra blood cell lysate
- 1 November 1977
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 93 (2), 309-311
- https://doi.org/10.1002/jcp.1040930217
Abstract
Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic absorption spectroscopy. The results were unaffected by temperature from 4–21°C, and by ionic strength in the range 0.09‐1.0 M (NaCl). Appreciable loss of vanadium in the supernatant was experienced above pH 2.2. Experiments at pH 2.1 under anaerobic conditions show that the green chromogen and the vanadium‐containing bands elute separately. Under these experimental conditions, the vanadium‐containing species is of relatively low (⋍ 1,000) molecular weight.This publication has 6 references indexed in Scilit:
- THE BLOOD OF ASCIDIA NIGRA: BLOOD CELL FREQUENCY DISTRIBUTION, MORPHOLOGY, AND THE DISTRIBUTION AND VALENCE OF VANADIUM IN LIVING BLOOD CELLSThe Biological Bulletin, 1976
- Nuclear magnetic resonance spectrum of living tunicate blood cells and the structure of the native vanadium chromogen.Proceedings of the National Academy of Sciences, 1975
- THE VANADIUM AND SELECTED METAL CONTENTS OF SOME ASCIDIANSThe Biological Bulletin, 1974
- Vanadium content of some tunicatesComparative Biochemistry and Physiology, 1963
- On the nature of vanadium in vanadocyte hemolyzate from ascidiansArchives of Biochemistry and Biophysics, 1954
- Observations On the Blood of Certain Ascidians, with Special Reference to the Biochemistry of VanadiumJournal of Experimental Biology, 1939