An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues
Open Access
- 3 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 250 (2), 596-600
- https://doi.org/10.1016/0014-5793(89)80803-8
Abstract
The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1–2 nMadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 μMadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.Keywords
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