Serum Levels of Luteinizing Hormone and Gonadotropin-Releasing Hormone in Cycling, Castrated and Anestrous Ewes1

Abstract

Blood samples were collected at 4-min intervals for 4 hr from 2 ewes on the day of estrus and 2 ewes castrated 90 and 120 days previously. Serum levels of luteinizing hormone (LH) and gonadotropin-releasing hormone (Gn-RH) were determined by double antibody radioimmunoassays. Samples were collected during a portion of the preovulatory LH surge in each ewe with LH levels ranging from Endocrinology94: 713, 1974)