ZO-1 and cingulin: tight junction proteins with distinct identities and localizations
- 1 October 1989
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 257 (4), C621-C628
- https://doi.org/10.1152/ajpcell.1989.257.4.c621
Abstract
The relative localization of ZO-1 and cingulin, the only two known components of the tight junction, was compared in Madin-Darby canine kidney (MDCK) cells, chicken small intestine, rat kidney distal convoluted tubule, and a hepatoma cell line. Immunoblot analysis demonstrated that cingulin and ZO-1 are immunologically unrelated and that, in the colon, cingulin is a single polypeptide with a molecular mass of 140 kDa. Immunofluorescent localization of cingulin and ZO-1 in confluent monolayers of MDCK cells showed identical staining patterns. However, subconfluent MDCK cells showed distinct localizations of the two proteins. Both cingulin and ZO-1 were found at the plasma membrane only at areas of cell-cell contact, but cingulin was diffusely distributed within the cytoplasm, whereas ZO-1 showed a more clustered internal arrangement. Cingulin and ZO-1 were identically localized at the plasma membrane of hepatoma tissue culture (HTC) cells at sites of cell-cell contact. In chicken intestine examined at the ultrastructural level, immunogold particles associated with cingulin were found approximately three times farther from the junctional membrane than those affiliated with ZO-1.This publication has 17 references indexed in Scilit:
- Tight junction structure and ZO-1 content are identical in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance.The Journal of cell biology, 1988
- Cingulin, a new peripheral component of tight junctionsNature, 1988
- Characterization of ZO-1, a protein component of the tight junction from mouse liver and Madin-Darby canine kidney cells.The Journal of cell biology, 1988
- Zonulae occludentes in junctional complex-enriched fractions from mouse liver: preliminary morphological and biochemical characterization.The Journal of cell biology, 1984
- Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocelluloseGene Analysis Techniques, 1984
- Yeast RNA Polymerase II Genes: Isolation with Antibody ProbesScience, 1983
- Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro.The Journal of cell biology, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPYJournal of Histochemistry & Cytochemistry, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970