Lysomotropic amines cause intracellular accumulation of receptors for epidermal growth factor.
- 1 June 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (6), 3283-3287
- https://doi.org/10.1073/pnas.77.6.3283
Abstract
The process of internalization of receptors for [mouse] epidermal growth factor (EGF) occurs even without EGF stimulation and is not prevented by the lysomotropic agents methylamine or chloroquine. These agents inhibit the degradation of 125I-labeled EGF, thus preventing the rapid dissociation of EGF from cells. Furthermore, 125I-labeled EGF incubated with cells in the presence of methylamine becomes increasingly insensitive to trypsin with time, suggesting that the EGF receptor internalization is not prevented by alkylamines, but that there is an intracellular accumulation of ligand-receptor complex due to the loss of normal modes of ligand-induced receptor processing. Lysis of cells treated with methylamine results in recovery of 125I-labeled EGF binding. Fractionation of these lysates on sucrose density gradients demonstrates that EGF receptors are localized within membrane fractions having higher densities than fractions from lysates untreated cells.This publication has 28 references indexed in Scilit:
- Receptor-mediated pinocytosis of mannose glycoconjugates by macrophages: Characterization and evidence for receptor recyclingCell, 1980
- Inhibitory effect of ammonium chloride and chloroquine on the entry of the toxic lectin modeccin into HeLa cellsBiochemical and Biophysical Research Communications, 1979
- Coated pits, coated vesicles, and receptor-mediated endocytosisNature, 1979
- Direct visualization of the binding and internalization of a ferritin conjugate of epidermal growth factor in human carcinoma cells A-431.The Journal of cell biology, 1979
- Inhibition of receptor-mediated uptake of a lysosomal enzyme into fibroblasts by chloroquine, procaine and ammoniaExperimental Cell Research, 1979
- Internalization and processing of the EGF receptor in the induction of DNA synthesis in cultured fibroblasts: The endocytic activation hypothesisJournal of Supramolecular Structure, 1979
- Epidermal growth factor: Biological activity requires persistent occupation of high-affinity cell surface receptorsProceedings of the National Academy of Sciences, 1978
- A mutation that impairs the ability of lipoprotein receptors to localise in coated pits on the cell surface of human fibroblastsNature, 1977
- 125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts.The Journal of cell biology, 1976
- Initiation of 3T3 fibroblast cell division by epidermal growth factorJournal of Cellular Physiology, 1975