Fluorimetric measurement of adrenocortical steroids in concentrated acid solution

Publisher Website
Google Scholar
Abstract
Fluorophotometric methods of measure-ment of several adrenocortical steroids were developed, based upon the fluorogenic reaction of these steroids with very concentrated phosphoric or sulfuric acid. The influences of initial acid concentration, time and temperature of heating, contaminants and final dilution with water upon the fluorescence intensity were studied. Recommended procedures for the routine measurement of hydrocortisone, corticosterone and cortisone are given. Modifications of procedure are also suggested, to permit the fluorophotometric measurement of aldosterone, 11-deoxycorticosterone and other coricosteroids.