Glucosamine metabolism in regenerating rat liver
- 15 September 1976
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 158 (3), 589-592
- https://doi.org/10.1042/bj1580589
Abstract
1. Glycoprotein synthesis was investigated with [1-14C]glucosamine in vivo. [14C]Glucosamine was administered intravenously 24h after hepatectomy to rats. 2. Incorporation into the acid-soluble fraction was maximum at 15 min after injection both in sham-operated and hepatectomized rats. 3. Enhancement of incorporation into UDP-N-acetylhexosamine in regenerating liver was observed. However, its specific activity was lower, because of a greater enhancement of synthesis de novo of the amino sugar. 4. In the liver acid-insoluble fraction, maximum incorporation of [14C]glucosamine was at 30 min in sham-operated rats and 2 h in hepatectomized rats respectively. 5. In sham-operated rats, incorporation into the plasma acid-insoluble fraction followed that of the liver acid-insoluble fraction, but hepatectomy resulted in a rapid enchancement of incorporation into plasma. 6. It is concluded that synthesis of liver glycoproteins is stimulated after partial hepatectomy and that glycoproteins synthesized are released rapidly into the plasma.This publication has 13 references indexed in Scilit:
- Galactose metabolism in regenerating rat liverBiochemical Journal, 1976
- A proposed pathway of plasma glycoprotein synthesisMolecular and Cellular Biochemistry, 1975
- Formation of glucosamine 6-phosphate in regenerating rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1971
- Synthesis of intestinal glycoprotein. Incorporation of (I- 14 C) glucosamine in vitro.1971
- Plasma Protein Biosynthesis Following Laparotomy and Partial HepatectomyExperimental Biology and Medicine, 1970
- Studies on hog spleenBiochimica et Biophysica Acta (BBA) - Enzymology, 1970
- Liver Protein Synthesis after Partial Hepatectomy and Acute StressJournal of Biological Chemistry, 1967
- BIOSYNTHESIS OF GLYCOPROTEINS .3. GLUCOSAMINE INTERMEDIATES IN PLASMA GLYCOPROTEIN SYNTHESIS IN LIVERS OF PUROMYCIN-TREATED RATS1964
- BIOSYNTHESIS OF GLYCOPROTEINS .1. INCORPORATION OF GLUCOSAMINE-14C INTO LIVER + PLASMA PROTEINS OF RAT1964
- A MODIFIED COLORIMETRIC METHOD FOR THE ESTIMATION OF N-ACETYLAMINO SUGARSJournal of Biological Chemistry, 1955