In vitro Culture of Pyrodinium

Abstract

Japanese B encephalitis and some other arbor viruses were partially purified by cellulose column chromatography or by fluorocarbon deproteinization and tested for sensitivity to enzymes. Infectivity decreased markedly when the viruses were mixed with trypsin or pancreatic lipase at 37°C. The enzymes also impair the immunogenicity of the virus in rabbits. Poliovirus is resistant to the enzymes.