Mammalian cells contain a DNA-recombining activity that can be dissociated from topoisomerase activity
- 1 July 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (7), 527-533
- https://doi.org/10.1007/bf01115252
Abstract
The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian ceils and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase activity, they did not all contain DNA-recombining activity. A distinct, perhaps novel, enzyme may therefore promote DNA recombination in these cell-free systems.This publication has 21 references indexed in Scilit:
- Coprecipitation of topoisomerase activity with simian virus 40 nucleoprotein complexes by divalent cationsBiochimie, 1980
- Extracts of Drosophila embryos mediate chromatin assembly in vitro.Proceedings of the National Academy of Sciences, 1979
- Nicking-closing enzyme assembles nucleosome-like structures in vitro.Proceedings of the National Academy of Sciences, 1979
- Nicking-closing enzyme is associated with SV40 DNA in vivo as a sodium dodecyl sulfate-resistant complexNucleic Acids Research, 1979
- Proteins that Affect DNA ConformationAnnual Review of Biochemistry, 1978
- Renaturation of complementary single-stranded DNA circles: complete rewinding facilitated by the DNA untwisting enzyme.Proceedings of the National Academy of Sciences, 1977
- Assembly of SV40 chromatin in a cell-free system from Xenopus eggsCell, 1977
- Evidence for an intermediate with a single-strand break in the reaction catalyzed by the DNA untwisting enzyme.Proceedings of the National Academy of Sciences, 1976
- SV40 nucleoprotein complex activity unwinds superhelical turns in SV40 DNANature, 1974
- An Activity from Mammalian Cells That Untwists Superhelical DNA—A Possible Swivel For DNA ReplicationProceedings of the National Academy of Sciences, 1972