A method for the quantitative fractionation of mixtures of 2-methoxyoestrone, oestrone, ring D α-ketolic oestrogens, oestradiol-17β, 16-epioestriol and oestriol by partition chromatography and the Girard reaction
- 1 November 1960
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 77 (2), 400-406
- https://doi.org/10.1042/bj0770400
Abstract
A new chemical method which separates and quantitatively measures pure 2-methoxyestrone, estrone, 16[alpha]-hydroxyestrone (or 16-oxo-estradiol-17[beta]), estradiol-17)3, 17-epiestriol and estriol is described. Experimental conditions for the Girard reaction resulting in quantitative recoveries of these estrogens have been developed. A technique for the quantitative transfer of absorbed estrogen on Celite to a partition chromatogram is described. Partition columns for the separation of 2-methoxyestrone, estrone, 17[alpha]-hydroxy-RStrone (or 16-oxo-estradiol-17[beta]) and for the separation of estra-diol-17[beta], 16-epiestriol and estriol are described. The possible applications of this method are discussed.Keywords
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