Abstract
A new chemical method which separates and quantitatively measures pure 2-methoxyestrone, estrone, 16[alpha]-hydroxyestrone (or 16-oxo-estradiol-17[beta]), estradiol-17)3, 17-epiestriol and estriol is described. Experimental conditions for the Girard reaction resulting in quantitative recoveries of these estrogens have been developed. A technique for the quantitative transfer of absorbed estrogen on Celite to a partition chromatogram is described. Partition columns for the separation of 2-methoxyestrone, estrone, 17[alpha]-hydroxy-RStrone (or 16-oxo-estradiol-17[beta]) and for the separation of estra-diol-17[beta], 16-epiestriol and estriol are described. The possible applications of this method are discussed.