Nonsense suppressors of yeast cause osmotic-sensitive growth.
- 1 January 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (1), 305-309
- https://doi.org/10.1073/pnas.74.1.305
Abstract
Many nonsense suppressors of Saccharomyces cerevisiae cause growth inhibition on hypertonic media. Eight tyrosine-inserting UAA (ochre) suppressors, 8 tyrosine-inserting UAG (amber) suppressors, a leucine-inserting UAG suppressor and a serine-inserting recessive lethal UAG suppressor cause osmotic sensitivity, whereas a serine-inserting UAA suppressor does not cause sensitivity. Although the mechanism is not understood, the growth inhibition of specific suppressors on hypertonic media is correlated with their efficiencies of suppression. This heretofore unknown property of nonsense suppressors is useful for mitotic mapping, selecting tRNA mutants, selecting antisuppressors and scoring nonsense suppressors.Keywords
This publication has 19 references indexed in Scilit:
- Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitroCell, 1975
- Temperature-sensitive, osmotic-remedial mutants of Neurospora crassa: Osmotic pressure induced alterations of enzyme stabilityMolecular Genetics and Genomics, 1975
- Suppressors in YeastCurrent Topics in Microbiology and Immunology, 1974
- Tyrosine substitutions resulting from suppression of amber mutants of iso-1-cytochrome c in yeastJournal of Molecular Biology, 1973
- Amino acid replacements resulting from super-suppression of nonsense mutants of iso-1-cytochrome c from yeastJournal of Molecular Biology, 1971
- A recessive lethal super-suppressor mutation in yeast and other ψ phenomenaHeredity, 1971
- The Mutational Alteration of the Primary Structure of Yeast Iso-1-cytochrome cJournal of Biological Chemistry, 1968
- Repair of multiple defects of a regulatory mutant of neurospora by high osmotic pressure and by reversionArchives of Biochemistry and Biophysics, 1968
- Respiration-deficient mutants of yeastBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- STREPTOMYCIN, SUPPRESSION, AND THE CODEProceedings of the National Academy of Sciences, 1964